Sampling turf to identify diseases must be done carefully and according to the following three steps:

1. Collect a turf sample

2. Bag and label correctly

3. Send or deliver

It is most important when trying to identify a disease that the samples are taken from the most affected area. This does not mean areas that are already dead. The most affected area is usually at the edge where the turf still looks in reasonable condition.

For example, with a disease like rhizoctonia, which spreads in a circular fashion, do not sample from the middle of the circle but rather on the perimeter.

Take a large sample (minimum 50 mm plug) and ensure that all parts of the plant are included. That is, leaves, stolons, rhizomes and roots.

Samples must be sealed in a plastic bag so moisture is retained. Label the sample bag clearly with the:

• name of the business

• exactly where the sample was collected from

• the turf species

• date collected

• name of the sampler.

Remember that there may be other samples from the same business so the location is critical.

It's a good idea to use a waterproof marker so that the label cannot rub off.

Disease samples need to arrive at the testing laboratory as soon as possible. The causal agent will only survive as long as there is enough moisture in the plastic bag.

Therefore, try to choose a local laboratory experienced in turf diseases and check with them about their recommended method of transport and packaging.